Journal: Cancer letters

Article Title: DNA-PKcs promotes therapy resistance and metastatic recurrence in neuroblastoma

doi: 10.1016/j.canlet.2026.218383

Figure Lengend Snippet: (A, B) Correlation between PRKDC gene expression levels and NB tumor progression to stage 4. (C) Heatmap of differential expression of DNA replication and repair pathway (KEGG) genes in PTCs (green) at diagnosis and DTCs (red) at relapse. Color intensity represents expression level. Heatmap of KEGG pathways involved in DNA replication and repair in primary tumor cells (PTCs) at diagnosis (green) and in disseminated tumors cells (DTCs) at relapse (red). (D) PRKDC gene expression in PTCs at diagnosis, DTCs at diagnosis and in DTCs at relapse (Ambros study, n=86) revealed a significant upregulation of PRKDC in DTCs at relapse. (E) Western blot analysis in MYCN-amplified (BE(2)-C, LAN-1, IMR-32 and SK-N-DZ) and MYCN-non-amplified (SK-N-AS, SK-N-SH) NB cell lines (*p<0.05, ***p<0.001, ****p<0.0001).

Article Snippet: MYCN -amplified human NB cell lines (BE(2)-C (CVCL_V006), IMR-32 (CVCL_0346), LAN-1 (CVCL_1827) and SK-N-DZ (CVCL_1701)) and MYCN -non-amplified cells (SK-N-AS (CVCL_1700), and SK-N-SH (CVCL_0531)) were purchased from ATCC.

Techniques: Gene Expression, Quantitative Proteomics, Biomarker Discovery, Expressing, Western Blot, Amplification

Journal: bioRxiv

Article Title: Multi-omics analysis reveals integrin α3-dependent mechanisms of Zika virus oncolytic activity in pediatric neural tumors

doi: 10.64898/2026.05.01.722162

Figure Lengend Snippet: (A-B) Representative images at 8 dpi for SH-SY5Y (A) and IMR-32 (B) control tumorspheres or tumorspheres following infection with the ZIKV PRVABC59 or BeH819015 strain at an MOI of 2 or 10. (C-D) Quantification of tumorsphere area for SH-SY5Y (C) and IMR-32 (D) tumorspheres under the same conditions as in (A-B). Scale bar = 300 μm. N = 3, n ≥ 2. Statistical significance indicators (asterisks) are shown for comparisons at 8 dpi. (E-F) 3D viability of individual SH-SY5Y (E) and IMR-32 (F) tumorspheres infected with the ZIKV PRVABC59 strain at an MOI of 2 or 10. N = 3, n ≥ 3. (G-H) ZIKV titers in supernatants from individual SH-SY5Y (G) and IMR-32 (H) tumorspheres infected with the ZIKV PRVABC59 strain at an MOI of 2 or 10. N = 3, n ≥ 3. Different symbol shapes represent different biological replicates. D0, D2, D4, and D6 represent 0, 2, 4, and 6 dpi, respectively. Data are presented as mean ± SD. Statistical significance was calculated using two-way ANOVA with Holm-Šidák multiple comparisons test. * p < 0.05; ** p < 0.01; **** p < 0.0001. n.s. indicates no significance.

Article Snippet: Neuroblastoma SH-SY5Y (CRL-2266) and IMR-32 (CCL-127) cells were purchased from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Control, Infection

Journal: bioRxiv

Article Title: Multi-omics analysis reveals integrin α3-dependent mechanisms of Zika virus oncolytic activity in pediatric neural tumors

doi: 10.64898/2026.05.01.722162

Figure Lengend Snippet: (A-B) Volcano plots showing DEGs in SH-SY5Y (A) and IMR-32 (B) tumorspheres following infection with the ZIKV PRVABC59 strain at an MOI of 10. “padj” represents the adjusted p - value calculated using the Benjamini-Hochberg method. The dotted gray lines indicate the significance thresholds: -log 10 padj = 2 (equivalent to padj = 0.01). Red points represent significantly upregulated genes (log 2 fold change > 0 and -log 10 padj ≥ 2), while blue points represent significantly downregulated genes (log 2 fold change < 0 and -log 10 padj ≥ 2). Gray points represent genes that are not significantly differentially expressed. The top 30 upregulated or downregulated genes, ranked by -log 10 padj, are labelled with their gene symbols. (C) Heatmap showing significant KEGG pathways regulated by ZIKV infection in USP7, SH-SY5Y and IMR-32 models.

Article Snippet: Neuroblastoma SH-SY5Y (CRL-2266) and IMR-32 (CCL-127) cells were purchased from the American Type Culture Collection (ATCC, Manassas, USA).

Techniques: Infection